Nucleotide sequence of the psaC gene of the cyanobacterium Synechococcus vulcanus.
نویسندگان
چکیده
The psaC is found in chloroplast genomes of higher plants, tobacco (1), liverwort (2), barley (3), maize (4), pea (5) and wheat (5). It encodes a photosystem-I associated polypeptide, designated as Subunit VII, 9 kDa subunit, PsaC, etc., presumably the apoprotein for iron-sulfur clusters, Centers A and B. We previously reported a partial N-terminal amino acid sequence of Subunit VII of a thermophilic cyanobacterium, Synechococcus vulcanus (6). Here, we report the isolation and the nucleotide sequence of a Synechococcus genomic library cloned into lambdadash, which has homology to a tobacco psaC DNA probe. Nucleotide sequencing of a fragment revealed that the Synechococcus vulcanus psaC gene consists of 81 codons, the same length as the tobacco gene that we previously reported (1) as well as other higher plants (2-5). Some of the features include: the 3 5 box, 3 4 3 9 ; the 1 0 box, 5 7 6 2 ; the Shine—Dalgamo sequence, 115-119. The psaC gene starts from 130 and ends at 372. The deduced amino acid sequence indicates an 87.5% homology compared with the tobacco sequence. The nucleotide sequence homology is 73.2% of that of the tobacco gene. The GC content of this thermophilic prokaryote gene is 54.1 % which is considerably higher than those of mesophilic plants (less than 42%, calculated from ref. 1—5).
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 18 12 شماره
صفحات -
تاریخ انتشار 1990